RADIOISOTOPES

Online ISSN: 1884-4111 Print ISSN: 0033-8303
RADIOISOTOPESは日本アイソトープ協会が発行する学術論文誌です
Radioisotopes 71(3): 245-253 (2022)
doi:10.3769/radioisotopes.71.245

原著Article

エピガロカテキンガレートおよびエピカテキンによる細胞ならびにDNAの放射線防護効果に関する研究Radiation Protection of Yeast Cells and DNA by Epigallocatechin Gallate and Epicatechin

Thi Nhan Tran1，Lukas Wisnu Wicaksono2，Maradi Abdillah2，松尾 陽一郎3，清水 喜久雄4，久米 恭5，泉 佳伸4Thi Nhan Tran1, Lukas Wisnu Wicaksono2, Maradi Abdillah2, Youichirou Matuo3, Kikuo Shimizu4, Kyo Kume5, Yoshinobu Izumi4

1 ベトナム電力大学Electric Power University, Vietnam

2 インドネシア原子力規制庁Nuclear Energy Regulatory Agency (BAPETEN), Indonesia

3 福井大学学術研究院工学系部門原子力安全工学講座Department of Nuclear Power and Safety Engineering, University of Fukui, Japan

4 福井大学附属国際原子力工学研究所Research Institute of Nuclear Engineering, University of Fukui, Japan

5 若狭湾エネルギー研究センターThe Wakasa Wan Energy Research Center, Japan

受付日：2021年10月14日Received: October 14, 2021
受理日：2022年7月28日Accepted: July 28, 2022
発行日：2022年11月15日Published: November 15, 2022
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エピガロカテキンガレート（EGCG）及びエピカテキン（EC）は、天然の放射線防護剤の一種と考えられている。ガンマ線及びヘリウム粒子線を照射した場合の、EGCG及びEC添加による放射線防護効果について、プラスミドDNA（pUC118）及び出芽酵母の野生株を用いてDNA鎖切断及び生存率への効果を評価した。結果から、EGCG及びECを添加した場合、未損傷のプラスミドDNAの割合が上昇すること、及び生存率が上昇することが明らかになった。

Exposure to ionizing radiation results in the generation of reactive intermediate species such as cation radicals, anion radicals, and free radicals that can induce DNA damage. Epigallocatechin gallate (EGCG) and epicatechin (EC) are natural radiation-protective agents. The radiation protection effects of EGCG and EC against γ-ray and helium ion irradiation were studied using plasmid pUC118 and wild-type yeast. The percentage of strand breaks of pUC118 in the presence or absence of EGCG or EC were evaluated by agarose gel electrophoresis, and the protective effects of EGCG in yeast cells exposed to radiation were assessed by the survival ratio. The percentage of non-damaged plasmid DNA after irradiation increased in the presence of both EGCG and EC, indicating that these compounds can reduce DNA damage induced by γ-ray and helium ion irradiation. Our results show that EGCG and EC may provide effective protection against the harmful effects of ionizing radiation.

Key words: radiation; scavenger; epigallocatechin-gallate; epicatechin; LET; DNA; single-strand break

© Japan Radioisotope Association 2022. This is an open access article distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) License (https://creativecommons.org/licenses/by/4.0/)© Japan Radioisotope Association 2022. This is an open access article distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) License (https://creativecommons.org/licenses/by/4.0/)

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