Online ISSN: 1884-4111 Print ISSN: 0033-8303
Radioisotopes 71(3): 179-184 (2022)


植物の根におけるイオンの流入,流出,正味流量の測定法の確立Development of the Practical Method for Ion Influx, Efflux, and Net Flux Measurement in Plant Roots

1東京大学大学院Graduate School of Agricultural and Life Sciences, the University of Tokyo

2東北大学CYRICCyclotron and Radioisotope Center, Tohoku University

3量子科学技術研究開発機構National Institute for Quantum Science and Technology

4星薬科大学Hoshi University

受付日:2022年3月17日Received: March 17, 2022
受理日:2022年5月9日Accepted: May 9, 2022
発行日:2022年11月15日Published: November 15, 2022

変異体や品種間差の解析での利用を見据え,植物の根における各イオン流量(net flux,influx,efflux)の定量的測定法の確立を目指した。得られたflux値の整合性をMg2+とNa+について検討したところ,イネの根ではトレーサー吸収時間20秒でinflux値が,15分以上でnet flux値が得られることがわかった。さらに,efflux値の測定ではトレーサー標識時間を最大限長くすることの重要性が示唆された。

Tracer experiments using radioisotope are powerful for the analysis of mineral uptake mechanisms in plant roots. In this study, we aimed to establish a quantitative method for measuring ion fluxes, i.e. net flux, influx, and efflux, which can be used to phenotyping of the mutants and natural variations. Consistency of obtained flux values for Mg2+ and Na+ was examined, and it was found that radioisotope absorption time of 20 s gave influx rate and an absorption time of more than 15 min gave net flux rate in rice roots. Furthermore, it was suggested that the longest possible radioisotope labeling time is necessary to increase the accuracy of the calculated efflux rate.

Key words: plant nutrition; ion transport; rice; tracer experiment; influx; efflux; net flux

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