Online ISSN: 1884-4111 Print ISSN: 0033-8303
Radioisotopes 68(5): 339-344 (2019)


電子線グラフト重合法によるPoly(N-isopropylacrylamide)を導入した温度応答性細胞培養膜の作製Fabrication of Thermo-responsive Cell Culture Membrane with Poly(N-isopropylacrylamide) by Electron Beam Graft Polymerization

1早稲田大学理工学術院総合研究所Research Institute for Science and Engineering, Waseda University ◇ 169–8555 東京都新宿区大久保3–4–1 ◇ 3–4–1 Okubo, Shinjyuku-ku, Tokyo 169–8555, Japan

2大阪大学大学院工学研究科Graduate School of Engineering, Osaka University ◇ 565–0871 大阪府吹田市山田丘2–1 ◇ 2–1 Yamadaoka, Suita, Osaka 565–0871, Japan

受付日:2018年11月20日Received: November 20, 2018
受理日:2019年1月22日Accepted: January 22, 2019
発行日:2019年5月15日Published: May 15, 2019

培養細胞と体内組織の親和性向上,細胞培養時の立体的な方向制御のため,温度応答性細胞培養膜表面への微細構造導入を試みた。電子線グラフト重合法とµmオーダーの格子状Si-maskを用い,Ethylene-tetrafluoroethylene copolymer (ETFE)にPoly(N-isopropylacrylamide)(PNIPAAm)を部分付与した。共焦点レーザー顕微鏡により電子線拡散とグラフト鎖伸長による丸みのある3 µmの凹凸を観察し,顕微FT-IR測定により凹凸とPNIPAAm付加分布の対応を確認した。PNIPAAmの下限臨界溶解温度(LCST)前後の接触角評価から濡れ性が変化し,細胞接着剥離の制御性が示唆された。

Our research group has attempted to introduce a micropattern on the surface of thermo-responsive cell culture membranes in order to improve the affinity between cultured cells and body tissues and control the direction three-dimensionally. Therefore, Poly(N-isopropylacrylamide) (PNIPAAm) was partially grafted to Ethylene-tetrafluoroethylene copolymer (ETFE) by electron beam graft polymerization method under N2 gas atmosphere at room temperature, using a Si-made mask that is microfabricated with µm order grid shapes. Roundish micro level unevenness of approximately 3 µm, which is due to electron beam diffusion and graft chain elongation, could be observed on the surface by evaluation using a confocal laser microscope. Distribution of PNIPAAm corresponding to the uneven shape was confirmed by microscopic FT-IR. Moreover, wettability change due to temperature change was observed from evaluation of water contact angles around the lower critical solution temperature. From these result, controllability of cell attachment / detachment properties was suggested.

Key words: electron beam; graft-polymerization; poly(N-isopropylacrylamide); microfabrication; cell culture membrane

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