Online ISSN: 1884-4111 Print ISSN: 0033-8303
Radioisotopes 66(9): 367-382 (2017)

総説Review Article

1981年から2000年の間に植物の代謝における13C/12C, 15N/14N同位体分別の研究をどう展開したか,その後どう展開されたか?The 1981–2000 Studies of 13C/12C and 15N/14N Discrimination in the Metabolism of Higher Plants, and the Progress Since Then

1農業・食品産業技術総合研究機構フェローNational Agriculture and Food Research Organization Fellow ◇ 305–8666 茨城県つくば市観音台3–1–1 ◇ Kannondai 3–1–1, Tsukuba, Ibaraki 305–8666 Japan

2東京大学名誉教授Professor Emeritus, University of Tokyo ◇ 113–8657 東京都文京区弥生1–1–1 ◇ Yayoi 1–1–1, Bunkyo-ku, Tokyo 113–8657 Japan

発行日:2017年9月15日Published: September 15, 2017

1981年から2000年の筆者の13C/12C, 15N/14N同位体分別の研究では植物組織や代謝物など多数試料の精密分析を行った.成果は:(1)植物組織や篩管液のδ13C 値とそれらのN獲得や乾燥などによる変動,(2)植物による窒素の獲得,代謝,体内移行での15N/14N 同位体分別,(3)δ13Cとδ15Nトレーサー法の生態学的適用;日本のクロボク土壌生成やタイとフィリピンの森林からサトウキビ土壌への移行における植物残渣由来Cの集積と代謝の解析ならびに植物における各種窒素源からの吸収量の推定である.これらの研究がその後どのように展開されたかをレビューした.

During 1981–2000, my research focused on the precise determination of the natural abundances of the 13C/12C and 15N/14N ratios (δ13C, δ15N) in many samples of plant tissues and metabolites. That research benefited from the previous work performed by pioneers in this field. My studies during that period provided the followings; (1) the identification of significant differences in the δ13C values in plant tissues and phloem sap affected by biotic (N acquisition, plant species and cultivars) and abiotic (drought) factors, (2) the discrimination of 15N/14N during the uptake, metabolism, and translocation of nitrate, ammonium and biologically-fixed N, and (3) the application of δ13C and δ15N tracing techniques for the quantification of plant residue dynamics (i.e., accumulation and degradation) in soils, plant N uptake from different sources (soils, chemical fertilizers, organic manures) and field biological N2 fixation by legumes including trees and non-legumes such as sugarcane and sweet potato, respectively. Here I review my publications and findings which gave insight for a precise understanding of whole plant C/N metabolisms.

Key words: ecological C and N dynamics; isotope discrimination; natural 13C/12C abundance (δ13C); natural 15N/14N abundance (δ15N); plant metabolism

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