Online ISSN: 1884-4111 Print ISSN: 0033-8303
Radioisotopes 66(4): 137-148 (2017)


拡大投影型軟X線顕微鏡での像修正におけるノイズ除去の効果Effectiveness of Noise Removal for Image Correction in Soft X-ray Projection Microscopy

1千葉大学大学院融合科学研究科Graduate School of Advanced Integration Science, Chiba University ◇ 263–8522 千葉市稲毛区弥生町1–33 ◇ 1–33 Yayoi-cho, Inage-ku, Chiba-shi, Chiba Pref. 263–8522, Japan

2東海大学工学部原子力工学科School of Engineering, Tokai University ◇ 259–1292 神奈川県平塚市北金目4–1–1 ◇ 4–1–1 Kitakaname, Hiratsuka-shi, Kanagawa Pref. 259–1292, Japan

3都立産業技術研究センターTokyo Metropolitan Industrial Technology Research Institute ◇ 135–0064 東京都江東区青海2–4–10 ◇ 2–4–10 Aomi, Koto-ku, Tokyo 135–0064, Japan

受付日:2016年9月30日Received: September 30, 2016
受理日:2016年12月27日Accepted: December 27, 2016
発行日:2017年4月15日Published: April 15, 2017


Soft X-ray microscopy has been developed for high resolution imaging of hydrated biological specimens due to the availability of water window region. In particular, a projection type microscope has advantages in wide viewing area, easy zooming function and easy extensibility to CT. The blur of projection image due to the diffraction of X-rays, which eventually reduces spatial resolution, could be corrected by an iteration procedure, i.e., repetition of Fresnel and inverse Fresnel transformations. However, it was found that the correction is not sufficiently effective for all images, especially for images with low contrast. In this study, first, grayscale distribution of the projection images was analyzed to reveal the reason for the insufficient correction. The result showed that the insufficient correction is due to high background noises. Therefore, a noise removal method prior to the iteration procedure was applied and found to be effective. Thus, some chromosome images with magnification of 329 times or lower which had not been correctable solely by the iteration procedure became successfully correctable after noise removal in combination with our contrast enhancement method whose effect was evaluated as effective in the previous study.

Key words: soft X-ray; projection microscopy; iteration procedure; noise removal; image enhancement

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